Thermo Fisher Scientific Methylglyoxal Monoclonal Antibody (9F11), PE
Methylglyoxal 변형 단백질을 특이적으로 인식하는 단일클론 항체로, Western blot, ICC/IF, Flow cytometry, ELISA에 적합합니다. PE 형광 표지로 565/576 nm 파장에서 검출 가능하며, 단백질 G로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9F11 |
| Immunogen | Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932270 |
Additional Formats
- Unconjugated (MA5-27568)
- APC (MA5-45813)
- FITC (MA5-45814)
- PerCP (MA5-45815)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45816 was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Target Information
Methylglyoxal (pyruvaldehyde, 2-oxopropanal) is an organic compound with the formula CH₃C(O)CHO. It contains both aldehyde and ketone groups and exists as hydrates and oligomers in aqueous environments. It is a reduced derivative of pyruvic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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