Thermo Fisher Scientific Cytokeratin 6 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of human keratin 6 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 6,uniProtId:P02538-1,ncbiNodeId:9606,antigenRange:564,antigenLength:564,antigenImageFileName:PA5-32466_Cytokeratin_6_P02538-1_Rabbit.svg,antigenImageFileNamePDP:PA5-32466_Cytokeratin_6_P02538-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Cytokeratin 6,uniProtId:P48668-1,ncbiNodeId:9606,antigenRange:564,antigenLength:564,antigenImageFileName:PA5-32466_Cytokeratin_6_P48668-1_Rabbit.svg,antigenImageFileNamePDP:PA5-32466_Cytokeratin_6_P48668-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:Cytokeratin 6,uniProtId:P04259-1,ncbiNodeId:9606,antigenRange:564,antigenLength:564,antigenImageFileName:PA5-32466_Cytokeratin_6_P04259-1_Rabbit.svg,antigenImageFileNamePDP:PA5-32466_Cytokeratin_6_P04259-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2549935

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is prostate or skin tissue.

Target Information

Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.