Thermo Fisher Scientific Human BRAF, GST Tag Recombinant Protein
인간 BRAF 단백질을 GST 태그로 발현한 재조합 단백질로, 키나아제 활성 분석에 적합합니다. 고순도 정제된 액상 형태이며, -80°C에서 보관 시 안정성을 유지합니다. MAPK/ERK 신호전달 연구 및 암 관련 변이 연구용으로 사용됩니다.
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Thermo Fisher Scientific Human BRAF, GST Tag Recombinant Protein
Applications
- Kinase Assay (KA)
Tested Dilution
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Insect cells |
| Amino Acid Sequence | Full length |
| Tag | GST-tag |
| Molecular Weight | 113.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris, pH 7.5, with 50% glycerol, 0.02% Triton X-100, 150 mM NaCl, 0.5 mM EDTA, 2 mM DTT |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
- For maximum recovery, please spin prior to use.
- Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use material in original packaging until exhausted.
- For larger sizes, aliquoting may reduce freeze/thaw cycles; aliquots below 20 µL are not recommended.
- Never store kinase diluted.
- Properly stored at -80°C, product is guaranteed for 6 months from date of purchase.
- Protein Form: Full Length
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and plays a role in postsynaptic responses of hippocampal neurons. This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion.
Mutations in B-Raf have been associated with various cancers, including:
- Non-Hodgkin lymphoma
- Colorectal cancer
- Malignant melanoma
- Thyroid carcinoma
- Non-small cell lung carcinoma
- Adenocarcinoma of lung
A pseudogene located on chromosome X has been identified for B-Raf.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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