Thermo Fisher Scientific Human ROCK1, GST Tag Recombinant Protein

Thermo Fisher Scientific Human ROCK1, GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution

• Assay-dependent

Product Specifications

Product Specific Information

For maximum recovery, please spin prior to use.
Aliquots of the 5 µg, 10 µg, and 20 µg sizes are not recommended; use materials in original packaging until exhausted.
For larger sizes, reduce freeze/thaw cycles by preparing aliquots (not below 20 µL).
Never store kinase diluted.
Properly stored at -80°C, product is guaranteed for 6 months from date of purchase.

Protein Form: Recombinant, Catalytic

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration.
The Rho/ROCK pathway is involved in the progression of various human cancers.
ROCK1 is identified as a Caspase-3 substrate and contributes to myosin light chain (MLC) activation through:

• Direct phosphorylation of MLC
• Phosphorylation of MLC phosphatase, leading to inhibition

During apoptosis, ROCK1 is cleaved by caspase-3, removing its inhibitory domain and resulting in constitutive kinase activity.
This cleavage induces MLC phosphorylation and membrane blebbing, key events in apoptotic execution.

Usage Note

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.