Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (YTH862.2)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

1:50-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

YTH862.2

Immunogen

PHA activated human peripheral blood lymphocytes. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-ABC,uniProtId:P04439-1,ncbiNodeId:9606,antigenRange:1-365,antigenLength:365,antigenImageFileName:MA1-80014_HLA-ABC_P04439-1_Rat.svg,antigenImageFileNamePDP:MA1-80014_HLA-ABC_P04439-1_Rat_PDP.jpeg,sortOrder:1},{targetFamily:HLA-ABC,uniProtId:P01889-1,ncbiNodeId:9606,antigenRange:1-362,antigenLength:362,antigenImageFileName:MA1-80014_HLA-ABC_P01889-1_Rat.svg,antigenImageFileNamePDP:MA1-80014_HLA-ABC_P01889-1_Rat_PDP.jpeg,sortOrder:2},{targetFamily:HLA-ABC,uniProtId:P10321-1,ncbiNodeId:9606,antigenRange:1-366,antigenLength:366,antigenImageFileName:MA1-80014_HLA-ABC_P10321-1_Rat.svg,antigenImageFileNamePDP:MA1-80014_HLA-ABC_P10321-1_Rat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_931638

Product Specific Information

MA1-80014 recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain. Recent studies have shown that this antibody may be able to induce apoptosis in activated but not resting T cells. This antibody does not react with mouse.

This product contains sodium azide; removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes are suitable for this purpose.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.