Thermo Fisher Scientific LOK Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 적합한 Rabbit Polyclonal 항체. Human LOK 단백질(aa 28–302)에 대한 항원으로 제작. 1 mg/mL 농도의 액상 제품으로 항원 친화 크로마토그래피 정제. 단기 4°C, 장기 -20°C 보관 권장.
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- PA529404
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 28–302 of Human LOK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546880 |
Product Specific Information
- Recommended positive controls: A431, Raji, HeLa
- Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (82%), Xenopus laevis (84%), Chicken (90%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Ubiquitin:
Exists either covalently attached to another protein or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues (polyubiquitin chains), or a linear polymer linked via the initiator Met (linear polyubiquitin chains).
Polyubiquitin chains have different functions depending on the Lys residue linkage:
- Lys-6: DNA repair
- Lys-11: ERAD and cell-cycle regulation
- Lys-29: Lysosomal degradation
- Lys-33: Kinase modification
- Lys-48: Protein degradation via proteasome
- Lys-63: Endocytosis, DNA-damage responses, NF-kappa-B signaling
Linear polymer chains via Met attachment are involved in cell signaling.
Unanchored polyubiquitin also plays roles in activation of protein kinases and signaling.
60S ribosomal protein L40:
Component of the 60S ribosomal subunit, essential for translation of specific cellular transcripts, particularly cap-dependent translation of vesicular stomatitis virus mRNAs.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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