Thermo Fisher Scientific QKI Recombinant Rabbit Monoclonal Antibody (1D1N6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1D1N6

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 242-341 of human QKI (Q96PU8) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:QKI,uniProtId:Q96PU8-1,ncbiNodeId:9606,antigenRange:242-341,antigenLength:341,antigenImageFileName:MA5-42589_QKI_Q96PU8-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42589_QKI_Q96PU8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911730

Product Specific Information

Positive test controls include: SKOV3, NIH/3T3, U-251MG, Neuro-2a, Mouse brain, Rat brain, Rat heart. The target is usually found in the following locations: Cytoplasm, Nucleus.

Immunogen sequence: RTPTPAGPTI MPLIRQIQTA VMPNGTPHPT AAIVPPGPEA GLIYTPYEYP YTLAPATSIL EYPIEPSGVL GAVATKVRRH DMRVHPYQRI VTADRAATGN

Target Information

This gene encodes the sacsin protein, which includes a UbL domain at the N-terminus, a DnaJ domain, and a HEPN domain at the C-terminus. The gene is highly expressed in the central nervous system, also found in skin, skeletal muscles and at low levels in the pancreas. This gene includes a very large exon spanning more than 12.8 kb. Mutations in this gene result in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder characterized by early-onset cerebellar ataxia with spasticity and peripheral neuropathy. Sacsin has been found to protect against mutant ataxin-1. A pseudogene associated with this gene is located on chromosome 11. Alternative splicing of this gene results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.