Thermo Fisher Scientific IFN gamma Monoclonal Antibody (AN-18), eBioscience
마우스 IFN-γ를 인식하는 단클론 항체로 ELISA, IP, 기능 분석 등에 적합합니다. Rat IgG1, kappa 아이소타입이며 비결합형 액상 제품입니다. 0.5 mg/mL 농도로 제공되며 4°C 보관 조건을 권장합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Notes | Publications |
|---|---|---|
| ELISA | - | View 35 publications |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Functional Assay | - | View 2 publications |
| Radioimmune Assay (RIA) | - | View 1 publication |
| T-Cell Activation (TCA) | - | View 1 publication |
| Immunostaining (IS) | 0.25–1 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AN-18 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468471 |
Additional Formats
Product Specific Information
Description:
The AN-18 antibody reacts with mouse interferon-gamma (IFN-γ), a 15–20 kDa cytokine secreted by Th1 cells.
Applications Reported:
Reported for use in immunoprecipitation, ELISPOT, and ELISA.
For ELISPOT capture, use Functional Grade purified AN-18 (Product #16-7313).
Applications Tested:
Tested as capture antibody in sandwich ELISA for mouse IFN-γ detection, using biotin R4-6A2 (13-7312) for detection and recombinant mouse IFN-γ (39-8311) as standard.
Recommended capture concentration: 0.25–1 µg/mL.
Include a standard curve (200 pg/mL–1.6 pg/mL) in each ELISA plate.
Purity: Greater than 90% (SDS-PAGE)
Aggregation: Less than 10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
IFN gamma (Interferon gamma, Type II interferon)
A macrophage activation factor produced by T-lymphocytes and NK cells in response to antigens and cytokines.
- Dimeric protein (~45 kDa) consisting of two 146 amino acid subunits
- Appears as 25, 20, and 15.5 kDa bands on SDS-PAGE (due to differential glycosylation)
- Highly species-specific (no cross-reactivity between human and mouse IFN-γ)
- Functions include: antiviral activity, tumor antiproliferative effects, MHC induction, macrophage activation, and enhanced B cell immunoglobulin secretion
- Acts via IFN-γ receptor I/II and JAK-STAT pathway
- Upregulated by IL-2, FGF basic, EGF; downregulated by vitamin D3 or DMN
- Mutations associated with aplastic anemia
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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