Thermo Fisher Scientific IL-23 p19 Monoclonal Antibody (5B2), eBioscience
마우스 IL-23 p19 서브유닛 인식 단일클론 항체로, IL-23 샌드위치 ELISA의 캡처 항체로 사용됩니다. 비중화성 항체이며, 높은 순도(>90%)와 낮은 응집도(<10%)를 가집니다. PBS(pH 7.2) 용액 내 0.5 mg/mL 농도로 제공되며, 4°C에서 보관합니다.
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Thermo Fisher Scientific IL-23 p19 Monoclonal Antibody (5B2), eBioscience
Applications and Tested Dilution
- Application: ELISA (Sandwich ELISA)
- Tested Dilution: 1–4 µg/mL
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_842745 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The 5B2 antibody reacts with the p19 subunit of mouse IL-23. When combined with the C17.8 antibody, it enables a more sensitive IL-23 sandwich ELISA compared to previous assays. The 5B2 antibody is non-neutralizing.
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. The p19 subunit alone is biologically inactive. IL-23 interacts with IL-12Rβ1 and an additional receptor subunit, IL-23R, containing a STAT4 binding domain. IL-23 is secreted by activated mouse and human dendritic cells.
In mice, IL-23 differs functionally from IL-12. It does not induce significant IFN-γ production but promotes proliferation and IL-17 production in memory T cells. In humans, IL-23 induces both memory T-cell proliferation and moderate IFN-γ production compared to IL-12.
Applications Reported:
The anti-mouse p19 5B2 antibody is used as a capture antibody in mouse IL-23 sandwich ELISA, paired with anti-p40 C17.8 as the detector.
Applications Tested:
- Capture antibody in sandwich ELISA for mouse IL-23 (p19/p40)
- Use with biotinylated C17.8 antibody (Product #13-7123-81) for detection
- Recombinant mouse IL-23 (Product #14-8231-63) as standard
- Recommended concentration: 1–4 µg/mL
- Standard curve range: 1000 pg/mL – 8 pg/mL
For specific neutralization of mouse IL-23 activity (without affecting IL-12 p70), use the functional grade purified G23-8 antibody (Product #16-7232-81).
Target Information
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 linked with p19. The p19 subunit alone is inactive. IL-23 binds to IL-12Rβ1 and IL-23R, leading to activation of STAT4 signaling. It is secreted by activated dendritic cells in both mouse and human systems.
Mouse IL-23 promotes proliferation and IL-17 production in memory T cells but not naïve T cells, whereas IL-12 does not affect memory cells. Human IL-23 also induces memory T-cell proliferation and moderate IFN-γ production compared to IL-12.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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