Thermo Fisher Scientific Vimentin Monoclonal Antibody (V9), FITC, eBioscience
인간 Vimentin을 인식하는 V9 단클론 항체로 FITC 형광 표지. 면역형광, IHC, Flow Cytometry 등 다양한 응용에 적합. 개, 닭, 쥐 반응성. 4°C 암소 보관, 동결 금지. 연구용으로만 사용.
- 카탈로그번호
- 11-9897-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Vimentin Monoclonal Antibody (V9), FITC, eBioscience
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 6 publications |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 6 publications |
| Flow Cytometry (Flow) | - | 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V9 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598045 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- eFluor 570
- eFluor 615
- eFluor 660
- Request custom conjugation
Product Specific Information
The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa intermediate filament protein expressed in cells derived from the mesenchyme and specific populations such as radial glia, immature glial cells, and pancreatic precursor cells. It is a proposed marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Surface expression has been observed on activated macrophages, platelets, apoptotic T cells, and neutrophils.
This antibody also recognizes canine, rat, and chicken vimentin but does not recognize mouse vimentin.
Applications Reported:
Immunocytochemistry, immunohistochemistry (frozen and paraffin-embedded tissue sections).
Applications Tested:
Tested by immunofluorescent staining of paraformaldehyde-fixed and permeabilized cells. Recommended use ≤ 1 µg/mL. Titrate for optimal performance.
Excitation / Emission: 488 nm / 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. During development, vimentin is replaced by tissue-specific intermediate filament proteins. It is also implicated in steroid synthesis regulation through its role in lipid droplet transport to mitochondria. Phosphorylation of vimentin induces cytoskeletal changes facilitating cholesterol transport and steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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