Thermo Fisher Scientific MEK2 Monoclonal Antibody (7F5)
MEK2 단백질을 타겟으로 하는 Thermo Fisher Scientific의 마우스 단클론 항체(7F5)입니다. Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능하며, 인간, 마우스, 랫트 시료에 반응합니다. 연구용으로 MAP2K2 단백질 검출에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7F5 |
| Immunogen | Purified recombinant fragment of human MAP2K2 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11001595 |
Product Specific Information
MA5-15600 targets MAP2K2 in indirect ELISA, FACS, IF, and WB applications and shows reactivity with human, mouse, and rat samples.
The immunogen is a purified recombinant fragment of human MAP2K2 expressed in E. coli.
MA5-15600 detects MAP2K2, which has a predicted molecular weight of approximately 44 kDa.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction.
Mitogen-activated protein kinase 2 (MEK2 or MAPKK2) belongs to a family of tyrosine/threonine protein kinases that activate ERK1 and ERK2 by phosphorylating both residues within the TEY motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, providing a negative feedback mechanism that suppresses MEK1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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