Thermo Fisher Scientific Phospho-Cytokeratin 18 (Ser53) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of Cytokeratin 18 only when phosphorylated at Ser53.

Target Information

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 8.
Tissues from gastrointestinal tract, respiratory tract, urogenital tract, endocrine and exocrine tissues, and mesothelial cells are positive for cytokeratin 18.
Cytokeratin 18 is primarily found in non-squamous epithelia and is present in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas.
Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis.
Two transcript variants encoding the same protein have been identified for the cytokeratin 18 gene.
Cytokeratin subtype expression patterns assist in distinguishing different types of epithelial malignancies.
Cytokeratin antibodies are useful in differential diagnosis of tumors by immunohistochemistry, cytopathology, and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.