Thermo Fisher Scientific beta Tubulin Chimeric Recombinant Rabbit Monoclonal Antibody (S11B)

Thermo Fisher Scientific beta Tubulin Chimeric Recombinant Rabbit Monoclonal Antibody (S11B)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Specificity: Binds specifically to human beta-tubulin with no cross-reactivity to alpha-tubulin.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene can cause congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, and this phosphorylation inhibits tubulin incorporation into microtubules.

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of tubulin dimers (alpha and beta subunits, ~55 kDa each). They play key roles in chromosome segregation, intracellular transport, ciliary and flagellar motion, and structural support. Because beta-tubulin is ubiquitously expressed, it is frequently used as a loading control in protein detection assays such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.