Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), APC, eBioscience
Thermo Fisher Scientific의 CD80 (B7-1) Monoclonal Antibody (16-10A1), APC 형은 Flow Cytometry용으로 검증된 항체입니다. Mouse, Dog, Pig에 반응하며, 633–647 nm Red Laser에서 Excitation/Emission 651/660 nm 특성을 가집니다. 면역세포 활성화 연구에 적합합니다.
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Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), APC, eBioscience
Applications
- Reported Application: Flow Cytometry
- Tested Dilution: 0.06 µg/test
- View 109 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Mouse, Pig |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16-10A1 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469416 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- Biotin
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. It binds with high affinity to T cell surface antigens CD28 and CD152 (CTLA-4), playing a crucial role in T-B cell communication and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes
- Recommended ≤ 0.06 µg/test (in 100 µL final volume)
- Cell number: 10⁵–10⁸ cells/test (empirically determined)
- Careful titration recommended for optimal assay performance
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells and various lymphoma cell lines. It provides regulatory signals for T lymphocytes via CD28 and CTLA-4 interactions.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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