Thermo Fisher Scientific DIABLO Recombinant Rabbit Monoclonal Antibody (006)
Recombinant Rabbit Monoclonal Antibody로 높은 특이성과 재현성을 제공. Human SMAC/Diablo 단백질 인식. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용 가능. 보존제 무첨가로 민감한 실험에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 006 |
| Immunogen | Recombinant Human SMAC/Diablo protein (Ala56–Asp239) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785128 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
This antibody has specificity for Human SMAC/Diablo.
Target Information
DIABLO (Smac) is a mitochondrial protein that activates various forms of apoptosis, likely by neutralizing members of the IAP (Inhibitor of Apoptosis Protein) family. During apoptosis, Smac exits the mitochondria and enters the cytosol. Mitochondrial-mediated apoptosis is essential for animal development and tissue homeostasis, and alterations can lead to malignant disorders.
Upon apoptotic stimuli, mitochondrial proteins such as cytochrome c and Smac/DIABLO are released into the cytosol via distinct mechanisms. Smac/DIABLO also plays a key role in sensitizing cancer cells to apoptosis induced by immune or drug-related factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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