Thermo Fisher Scientific TNF alpha Monoclonal Antibody (E10042)

Applications

ELISA (ELISA)

• Tested Dilution: 1:25–1:3200
• View 2 publications

Neutralization (Neu)

• Tested Dilution: —
• View 1 publication

Product Specifications

Product Specific Information

• M2TNFAI targets TNF Alpha in ELISA applications and shows reactivity with Human samples.
• The immunogen is recombinant human TNF alpha from Pichia pastoris.
• Detects TNF Alpha with a predicted molecular weight of approximately 26 kDa.
• Reconstitute in 500 µL PBS to create a stock of 1 mg/mL.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers of two to five noncovalently linked units but shows a single 17 kDa band under non-reducing SDS-PAGE conditions. Knockout studies in mice suggest TNF alpha has neuroprotective functions and induces tumor necrosis in tumor-bearing mice. TNF alpha also plays roles in immune responses to bacterial, viral, parasitic, and fungal infections, and in tumor cell cytolysis, synergistic with interferon-gamma. It is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.