Thermo Fisher Scientific RAP1GAP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:2,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Immunogen sequence: AMGLSNKKPN TVSTSHSGSF APNNPDLAKA AGISLIVPGK SPTRKKSGPF GSRRSSAIGI ENIQEVQEKR ESPPAGQKTP DSGHVSQEPK SENSSTQSSP EMPTTKNRAE TAAQRAEALK DFSRSSSSAS SFASVVEETE GVDGEDTGLE SVSSSGTPHK RDSFIYSTWL EDSVSTTSGG SSPGPSRSPH PDAGKLGDPA CPEIKIQLEA SEQHMPQLGC
• Positive Samples: U-87MG, 293T, SGC-7901, LO2
• Cellular Location: Golgi apparatus membrane, Peripheral membrane protein

Target Information

Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z (a member of the Gi family of trimeric G proteins) and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit and attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney, and pancreas and may act as a signal integrator to coordinate Gz signaling and Rap1 signaling. A novel isoform, Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases GTP-bound Rap1, activating ERK/MAPK pathways.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.