Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet series, eFluor 506, NovaFluor Blue/Red/Violet/Yellow series, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710, Super Bright series.
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Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b, in association with integrin beta2 (CD18), is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It serves as a receptor for intercellular adhesion molecules (CD54, CD102, CD50) and iC3b, playing a critical role in cell-cell and cell-matrix interactions.

• Applications Reported: Flow cytometric analysis.
• Applications Tested: Pre-titrated and validated for flow cytometry on human peripheral blood cells.
  Recommended use: 5 µL (0.5 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).
• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD11b (Integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that forms a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
CD11b/CD18 acts as a receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating crucial adhesion processes.
Expression levels vary across tissues and increase during monocyte maturation. Dysregulation of CD11b is linked to systemic lupus erythematosus and related autoimmune conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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