Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), FITC, eBioscience
마우스 CD11b를 인식하는 M1/70 클론의 FITC 결합 단클론 항체. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 다양한 형광체 포맷으로 제공. 498/517 nm 파장에서 Excitation/Emission. 4°C 보관, 동결 금지.
- 카탈로그번호
- 11-0112-41
- 판매단위
- pk
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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 4 |
| Immunohistochemistry (IHC) | - | 47 |
| Immunohistochemistry (PFA fixed) | - | 4 |
| Immunohistochemistry (Frozen) | - | 3 |
| Immunocytochemistry (ICC/IF) | - | 20 |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | 471 |
| Immunoprecipitation (IP) | - | 1 |
| Neutralization (Neu) | - | 4 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Ferret, Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11042156 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 506, 660
- Functional Grade
- NovaFluor Blue 510–660
- NovaFluor Yellow 570–660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780
- Custom conjugation available
Product Specific Information
The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form the alphaMbeta2 integrin (Mac-1), binding to CD54 (ICAM-1), C3bi, and fibrinogen. It is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells. M1/70 also cross-reacts with human CD11b, enabling detection in human monocytes, granulocytes, and NK cell subsets.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for use at 5 µL (0.25 µg) per test with mouse splenocytes or bone marrow cells.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that forms a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 binds ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell-cell and cell-matrix interactions. Expression varies by cell type and tissue, and increases during monocyte maturation.
Research Use Only. Not for diagnostic use or resale without authorization.
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