Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), FITC, eBioscience
Mouse CD11c 인식하는 N418 클론의 FITC 결합 단일클론 항체. 유세포분석 등 다양한 응용에 적합하며, 0.25 µg/test로 최적화된 사용 가능. 488 nm/520 nm 형광 특성, 고순도 친화크로마토그래피 정제, 4°C 보관.
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- 판매단위
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Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 19 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 8 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 235 publications |
| Affinity Purification (AP) | - | View 1 publication |
| In vitro Assay (IV) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_464939 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–711)
- eFluor series (450–660)
- Functional Grade
- NovaFluor Blue/Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright series (436–780)
- Request custom conjugation available
Product Specific Information
The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed by dendritic cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion interactions.
N418 binds to CD11c on splenic dendritic cells in T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of mouse splenocytes. Recommended ≤0.25 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11, along with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed on B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
- CD11b: Expressed on monocytes, macrophages, NK cells, some B/T cells, granulocytes.
- CD11c: Expressed on monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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