Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), FITC, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), FITC, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–711)
• eFluor series (450–660)
• Functional Grade
• NovaFluor Blue/Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright series (436–780)
• Request custom conjugation available

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed by dendritic cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion interactions.
N418 binds to CD11c on splenic dendritic cells in T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of mouse splenocytes. Recommended ≤0.25 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11, along with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed on B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
• CD11b: Expressed on monocytes, macrophages, NK cells, some B/T cells, granulocytes.
• CD11c: Expressed on monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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