Thermo Fisher Scientific HYOU1 Monoclonal Antibody (6G7-2H5), APC
HYOU1 단백질을 인식하는 APC 결합 mouse monoclonal 항체. Western blot 및 ICC/IF에 적합하며, 세포 내 단백질 폴딩 및 스트레스 반응 연구에 활용 가능. 4°C 보관, 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6G7-2H5 |
| Immunogen | Raised against a synthetic peptide of human GRP170 |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
1 µg/mL of MA5-45342 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 170 kDa.
Target Information
HYOU1 belongs to the heat shock protein 70 family and plays an important role in protein folding and secretion in the endoplasmic reticulum (ER). Suppression of HYOU1 is associated with accelerated apoptosis, suggesting a cytoprotective role during hypoxia-induced stress. It is up-regulated in tumors, particularly breast tumors, and is linked to tumor invasiveness. In rat, the protein localizes to both the ER and mitochondria, indicating a potential housekeeping function in the cytosol.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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