Thermo Fisher Scientific TARC Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930891

Product Specific Information

AA Sequence of recombinant protein: ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TARC (CCL17). Anti-Human TARC (CCL17)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hTARC by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TARC (500-P97) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTARC.

Western Blot: To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTARC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P97BT-1MG will be provided as 2 x 500 µg

Target Information

TARC ligand (CCL17) belongs to the CC chemokine family. It is a small cytokine and the gene is located on q arm of chromosome 16, in humans, along with other chemokines. TARC specifically binds to T-cells and interacts with chemokine receptors CCR4 and CCR8. It plays an important role in T cell development in thymus as well as in trafficking and activation of mature T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.