Thermo Fisher Scientific CXCL10 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific CXCL10 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IP-10 (CXCL10). Anti-Human IP-10 (CXCL10)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIP-10 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IP-10 (500-P93) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hIP-10.

Western Blot:
To detect hIP-10 by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hIP-10 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P93BT-1MG will be provided as 2 × 500 µg.

Target Information

IP-10 (interferon-gamma induced protein 10 kD; in mouse also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene belonging to the CXC chemokine superfamily. It was first cloned in 1985.
IP-10 has no activity on neutrophils but stimulates monocyte, NK, and T-cell migration, regulates T-cell and bone marrow progenitor maturation, modulates adhesion molecule expression, and inhibits angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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