Thermo Fisher Scientific CXCL10 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

View 4 publications 4 publications

In vitro Assay (IV)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IP-10 (CXCL10) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL10 (IP-10),uniProtId:P02778-1,ncbiNodeId:9606,antigenRange:22-98,antigenLength:98,antigenImageFileName:500-P93BT-25UG_CXCL10_IP-10_P02778-1_Rabbit.svg,antigenImageFileNamePDP:500-P93BT-25UG_CXCL10_IP-10_P02778-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930867

Product Specific Information

AA Sequence of recombinant protein: VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IP-10 (CXCL10). Anti-Human IP-10 (CXCL10)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hIP-10 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IP-10 (500-P93) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIP-10.

Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P93BT-1MG will be provided as 2 x 500 µg

Target Information

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.