Thermo Fisher Scientific CXCL10 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Human CXCL10 항체로, ELISA 및 Western blot에 최적화되어 높은 민감도로 IP-10 검출 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 -20°C에 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | View 4 publications |
| In vitro Assay (IV) | – | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IP-10 (CXCL10) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930867 |
Product Specific Information
AA Sequence of recombinant protein:
VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IP-10 (CXCL10). The antibody was purified by affinity chromatography and biotinylated.
Sandwich ELISA:
For hIP-10 detection by sandwich ELISA (100 µL/well), use 0.25–1.0 µg/mL of this antibody. In combination with PeproTech Polyclonal Anti-Human IP-10 (500-P93) as a capture antibody, it enables detection of 0.2–0.4 ng/well of recombinant hIP-10.
Western Blot:
For hIP-10 detection by Western blot, use 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P93BT-1MG is provided as 2 × 500 µg.
Target Information
IP-10 (interferon-gamma induced protein 10 kD; in mouse also called cytokine responsive gene 2, Crg-2, or mob-1) belongs to the CXC chemokine superfamily. It was first cloned in 1985.
IP-10 functions include:
- Stimulation of monocyte, NK, and T-cell migration
- Regulation of T-cell and bone marrow progenitor maturation
- Modulation of adhesion molecule expression
- Inhibition of angiogenesis
Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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