Thermo Fisher Scientific Calcineurin B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein of human Calcineurin B.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2719858

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits; calcineurin A (CnA), a 60 kDa catalytic subunit which is highly homologous to protein phosphatases 1 and 2A; and a 19 kDa regulatory subunit, calcineurin B (CnB) which, like calmodulin, contains four EF-hand calcium binding sites. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. The immunosuppressive drugs, cyclosporin A and FK506, when bound to the immunophilins, cyclophilin and FKBP respectively, inhibit dephosphorylation and activation of NF-AT by calcineurin. This inhibition is caused by the immunosuppressant/immunophilin complex simultaneously binding to both CnA and CnB which does not inhibit the phosphatase activity of CnA, but rather blocks access of the substrate to the catalytic domain of CnA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.