Thermo Fisher Scientific Calcineurin B Polyclonal Antibody
Calcineurin B 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, 고순도 항원 친화 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Calcineurin B Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein of human Calcineurin B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2719858 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
Target Information
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway.
Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits:
- Calcineurin A (CnA): 60 kDa catalytic subunit, homologous to protein phosphatases 1 and 2A
- Calcineurin B (CnB): 19 kDa regulatory subunit containing four EF-hand calcium binding sites
Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells.
Immunosuppressive drugs such as cyclosporin A and FK506, when bound to immunophilins (cyclophilin and FKBP respectively), inhibit NF-AT activation by calcineurin. This inhibition occurs as the immunosuppressant/immunophilin complex binds simultaneously to both CnA and CnB, blocking substrate access to the catalytic domain of CnA without inhibiting its phosphatase activity.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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