Thermo Fisher Scientific Calcineurin B Polyclonal Antibody

Thermo Fisher Scientific Calcineurin B Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway.
Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits:

• Calcineurin A (CnA): 60 kDa catalytic subunit, homologous to protein phosphatases 1 and 2A
• Calcineurin B (CnB): 19 kDa regulatory subunit containing four EF-hand calcium binding sites

Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells.
Immunosuppressive drugs such as cyclosporin A and FK506, when bound to immunophilins (cyclophilin and FKBP respectively), inhibit NF-AT activation by calcineurin. This inhibition occurs as the immunosuppressant/immunophilin complex binds simultaneously to both CnA and CnB, blocking substrate access to the catalytic domain of CnA without inhibiting its phosphatase activity.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.