Thermo Fisher Scientific MDM2 Monoclonal Antibody (OTI15C5), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability. Located on chromosome 12q, MDM2 phosphorylation by Akt at Ser166 and Ser186 enhances its interaction with p300, promoting p53 ubiquitination and degradation.
Phosphorylation also prevents binding to p19ARF, further increasing p53 degradation. MDM2 negatively regulates p53 function by inhibiting its transactivation role, and overexpression can reduce tumor suppressor activity.
MDM2 also possesses E3 ubiquitin ligase activity, contributing to p53 degradation. It is involved in apoptosis and cell cycle regulation, and is overexpressed in various human cancers including soft tissue carcinomas and breast cancer.
Beyond p53, MDM2 interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, influencing tumorigenesis. Over 40 alternatively spliced variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.