Thermo Fisher Scientific RGMA Polyclonal Antibody
Thermo Fisher Scientific의 RGMA Polyclonal Antibody는 Mouse와 Rat 시료에 반응하며, Western blot 및 IHC에 사용 가능합니다. Rabbit에서 유래한 Polyclonal 항체로, c-terminal RGMA 펩타이드를 면역원으로 제작되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous (PubMed) | - | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the C-terminal region of mature mouse Repulsive Guidance Molecule A (RGMA) conjugated to an immunogenic carrier protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: RGMA.
Target Information
RGMA is a member of the repulsive guidance molecule (RGM) family involved in multiple functions in the developing and adult nervous system. It regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and contributes to mature synapse formation.
Binding to its receptor NEO1/neogenin activates the RHOA-ROCK1/Rho-kinase signaling pathway through the UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to neuronal growth cone collapse and neurite inhibition.
Additionally, RGMA-NEO1 interaction affects HRAS inactivation via the HRAS-PTK2/FAK1-AKT1 pathway. RGMA also functions as a bone morphogenetic protein (BMP) coreceptor, signaling through SMAD1, SMAD5, and SMAD8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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