Thermo Fisher Scientific PCNA Polyclonal Antibody
Thermo Fisher Scientific의 PCNA Polyclonal Antibody는 인간 PCNA 단백질을 인식하는 토끼 IgG 항체로, 세포 증식 및 DNA 복제 연구에 적합합니다. Western blot과 IHC(P)에서 검증되었으며, 핵 내 PCNA 검출에 유용합니다. 항원 친화 크로마토그래피로 정제된 고품질 연구용 시약입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant human PCNA protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Tonsil, lymph node, or small intestine
- Cellular location: Nuclear
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and plays a key role in DNA excision and mismatch repair pathways. It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and is relevant to tumor prognosis. It is expressed in the nucleus, functions as a cofactor of DNA polymerase delta, and forms a homotrimer to enhance leading strand synthesis during replication.
In response to DNA damage, PCNA undergoes ubiquitination and participates in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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