Thermo Fisher Scientific Aggrecan Monoclonal Antibody (BC-3)
Aggrecan 단백질의 neoepitope(BC-3 클론)을 특이적으로 인식하는 Thermo Fisher monoclonal 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. 다종(인간, 설치류, 가축 등)에 반응하며, 단백질 분해 연구 및 연골 조직 분석에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | 19 publications |
| Immunohistochemistry (IHC) | – | 11 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:2,000 | 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:10–1:2,000 | 3 publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | 8 publications |
| Flow Cytometry (Flow) | – | 1 publication |
| ELISA | 1:1,000–1:5,000 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Horse, Cat, Guinea pig, Human, Sheep, Pig, Rabbit, Rat |
| Published Species | Bacteria, Human, Mouse, Rabbit, Rat, Zebrafish |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BC-3 |
| Immunogen | Synthetic peptide corresponding to human aggrecan ARGSV neoepitope |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568440 |
Product Specific Information
Purified recombinant Aggrecan Interglobular Domain (IGD) protein (amino acids T331–G458 with C-terminal His-tag, expressed in E. coli) was used to validate this antibody.
The recombinant protein contains cleavage sites for aggrecanases (E373–A374 in aggrecan) and matrix metalloproteinases (N341–F342 in aggrecan).
The recombinant protein was cleaved in vitro using recombinant Aggrecanase. Degradation of IGD and accumulation of cleaved fragments were visualized via MEMCode reversible protein staining, followed by Western blot using the BC-3 antibody for specific detection of the aggrecan neoepitope.
The antibody also recognizes full-length IGD (shown by ELISA) but with significantly lower affinity.
MA3-16888 has been successfully used in ELISA, immunohistochemistry (frozen and FFPE), and Western blot applications.
This antibody may be used to immunostain formalin-fixed, paraffin-embedded sections, alcohol-fixed frozen sections, and un-fixed snap-frozen sections.
For optimal epitope recognition, samples must be deglycosylated using:
- 0.01 Units Chondroitinase ABC
- 0.01 Units Keratanase
- 0.0001 Units Keratanase II per 10 µg S-GAG of non-deglycosylated aggrecan
MA3-16888 detects aggrecan in human, bovine, feline, canine, guinea pig, equine, porcine, rabbit, rat, and sheep samples.
Target Information
Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is encoded by the ACAN gene.
It is a major component of the extracellular matrix in cartilage, providing resistance to compression.
The human protein (~250 kDa, 2316 amino acids) exists in multiple isoforms due to alternative splicing.
Aggrecan exhibits a bottlebrush structure with chondroitin sulfate and keratan sulfate chains attached to an extended core protein.
Its synthesis and degradation are key processes in cartilage deterioration during joint injury, disease, and aging.
Aggrecan contains three interglobular domains (G1, G2, G3) involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis.
Fragments of aggrecan released into synovial fluid during osteoarthritis result from cleavage by aggrecanase and MMP activity.
Antibodies BC-3 and BC-4 detect neoepitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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