Thermo Fisher Scientific SHH Monoclonal Antibody (8G3)

Thermo Fisher Scientific SHH Monoclonal Antibody (8G3)

Applications and Tested Dilutions

• Western Blot (WB): 1:500–1:2,000
• ELISA: 1:10,000

Product Specifications

Product Specific Information

MA5-15647 targets SHH in indirect ELISA and Western blot applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human SHH expressed in E. coli.
MA5-15647 detects SHH, which has a predicted molecular weight of approximately 49.6 kDa.

Target Information

Sonic Hedgehog (SHH) is expressed only during embryogenesis and plays a critical role in early embryo patterning. It is a key inductive signal in the development of the ventral neural tube, anterior-posterior limb axis, and ventral somites.
SHH is produced as a precursor that undergoes autocatalytic cleavage:

• The N-terminal portion is soluble and responsible for signaling activity.
• The C-terminal portion aids in precursor processing and attaches a cholesterol moiety to the N-terminal product, restricting it to the cell surface.

Defects in SHH or its signaling pathway cause holoprosencephaly (HPE), where the forebrain fails to separate properly, resulting in facial deformities.
Mutations may also be associated with VACTERL syndrome, characterized by vertebral, anal, tracheoesophageal, renal, cardiac, and limb anomalies.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.