Thermo Fisher Scientific STAT3 Monoclonal Antibody (3B5)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-15712 targets STAT3 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples.
The immunogen is a purified recombinant fragment of human STAT3 expressed in E. coli.
MA5-15712 detects STAT3 with a predicted molecular weight of approximately 88 kDa.

Target Information

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor-associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors.
Coactivators such as CREB-binding protein are required for transcriptional activation by STAT3. STAT3 can be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF, and IL6.
Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity.
In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 mediates expression of various genes involved in cell growth and apoptosis.
Rac1 has been shown to bind and regulate STAT3 activity, while PIAS3 acts as a specific inhibitor.
Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described.
Deletion of the STAT3 gene in knock-out mice is lethal at the early embryonic stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.