Thermo Fisher Scientific Phospho-SMC1 (Ser966) Polyclonal Antibody
Phospho-SMC1 (Ser966) 폴리클로날 항체로, 인산화된 SMC1 단백질 검출에 최적화됨. Western blot, IHC, ICC, IP 등 다양한 응용 가능. Rabbit IgG 기반, 액상 형태로 제공. 세포 및 조직 내 염색에 높은 특이성과 재현성 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| Immunoprecipitation (IP) | 2–6 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phosphorylated synthetic peptide representing a portion of Human Structural Maintenance of Chromosomes 1 around serine 966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Notes:
- For ICC, formaldehyde fixation is recommended.
- Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Structural Maintenance of Chromosomes (SMC) family proteins are essential for nuclear events involving chromosome structural changes, including mitotic organization, DNA recombination and repair, and transcriptional repression.
SMC1 and SMC3 form a heterodimeric complex required for metaphase progression and sister chromatid cohesion.
Mutations in SMC1 can lead to Cornelia de Lange syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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