
Thermo Fisher Scientific Phospho-SMC1 (Ser966) Polyclonal Antibody
인간 SMC1 Ser966 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| Immunoprecipitation (IP) | 2–6 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phosphorylated synthetic peptide representing a portion of Human Structural Maintenance of Chromosomes 1 around serine 966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note:
- For ICC, formaldehyde fixation is recommended.
- Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Structural Maintenance of Chromosomes (SMC) family proteins are essential for nuclear processes involving chromosome structural changes, such as mitotic organization, DNA recombination and repair, and transcriptional repression.
SMC1 and SMC3 form a heterodimeric complex necessary for metaphase progression and sister chromatid cohesion. Mutations in the SMC1 gene are associated with Cornelia de Lange syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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