Thermo Fisher Scientific SIRT1 Monoclonal Antibody (3H10A10)
Human SIRT1 단백질을 인식하는 Mouse monoclonal antibody로, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능. 보존제가 없는 액상 형태이며 Protein A로 정제됨. 단기 보관은 4°C, 장기 보관은 -20°C에서 권장.
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Thermo Fisher Scientific SIRT1 Monoclonal Antibody (3H10A10)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| ELISA | 1:1,000–1:2,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H10A10 |
| Immunogen | Recombinant Human SIRT1 protein (Met193–Ser747) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785412 |
Product Specific Information
- This product is preservative-free.
- It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.
- This antibody has specificity for Human SIRT1.
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and coordinates multiple cellular functions such as cell cycle regulation, DNA damage response, metabolism, apoptosis, and autophagy.
SIRT1 modulates chromatin function through histone deacetylation and affects histone and DNA methylation, leading to transcriptional repression.
It is essential in skeletal muscle cell differentiation and mediates nutrient-dependent inhibition of myoblast differentiation involving AMPK and NAMPT pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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