Thermo Fisher Scientific PCNA Polyclonal Antibody
Rabbit polyclonal antibody targeting human PCNA for detection in WB, IHC, ICC, and IP applications. Reacts with multiple species including human, mouse, and rat. High purity via antigen affinity chromatography and suitable for cell proliferation studies.
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Thermo Fisher Scientific PCNA Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 7 publications |
| Immunohistochemistry (IHC) | – | View 9 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | View 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1–253 of Human PCNA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.11 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7) with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C (short term); for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2544690 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, PC-12, A549, SiHa
- Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (92%), Xenopus laevis (89%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA serves as a marker for proliferating cells and tumor prognosis, being expressed in early G1 and S phases. Located in the nucleus, PCNA acts as a homotrimeric cofactor for DNA polymerase delta, enhancing replication efficiency. Upon DNA damage, PCNA undergoes ubiquitination and participates in the RAD6-dependent repair pathway. Two transcript variants encode the same protein, with pseudogenes found on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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