Thermo Fisher Scientific MCL-1 Monoclonal Antibody (8C6, 8C6D4B1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-15236 targets MCL-1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with human samples.
The MA5-15236 immunogen is purified recombinant fragment of human MCL-1 expressed in E. coli.
MA5-15236 detects MCL-1, which has a predicted molecular weight of approximately 37 kDa.

Target Information

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in differentiation and maintenance of cell viability. Isoform 1 inhibits apoptosis, while isoform 2 promotes it. The C-terminal regions of MCL1 and Bcl-2 share significant sequence homology.
Expression of MCL1 increases upon exposure of ML-1 cells to DNA damaging agents such as ionizing radiation, ultraviolet radiation, and alkylating drugs. This is accompanied by increases in GADD45 and Bax and a decrease in Bcl-2.
Enhanced expression of MCL1, associated with mitochondria, complements Bcl-2 expression during ML-1 cell differentiation. MCL1 promotes cell viability under conditions that otherwise induce apoptosis, possibly by heterodimerizing with pro-apoptotic Bcl-2 family members such as Bim or Bak.
MCL1 is expressed in multiple cell types and is essential for embryogenesis and lymphocyte development. It exists as at least two isoforms, MCL1L and MCL1S, with MCL1S promoting cell death when overexpressed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.