Thermo Fisher Scientific Neuroserpin Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Recommended dilution: 0.1–0.2 mg/mL

ELISA

• Recommended dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Neuroserpin. Anti-Human Neuroserpin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human Neuroserpin by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Neuroserpin (500-P271) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human Neuroserpin.

Western Blot:
To detect Human Neuroserpin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 mg/mL. When used with compatible development reagents, the detection limit for Recombinant Human Neuroserpin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P271BT-1MG will be provided as 2 × 500 µg.

Target Information

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain and preferentially reacts with and inhibits tissue-type plasminogen activator. It plays a role in the regulation of axonal growth and synaptic plasticity. Mutations in this gene cause familial encephalopathy with neuroserpin inclusion bodies (FENIB), a dominantly inherited form of encephalopathy and epilepsy characterized by accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants encoding the same protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.