Thermo Fisher Scientific PECAM1 Monoclonal Antibody (OTI1G6), TrueMAB
PECAM1(CD31) 단백질을 인식하는 Mouse Monoclonal Antibody. WB, IHC, ICC, Flow 등 다양한 응용 가능. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 인체 세포에서 발현된 full-length PECAM1 항원으로 면역화됨.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G6 |
| Immunogen | Full-length human recombinant PECAM1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD31 (PECAM-1, platelet endothelial cell adhesion molecule-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs. It is expressed throughout the vascular compartment, mainly at intercellular junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
CD31 plays diverse roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, autoimmunity, phagocytosis, anaphylaxis, and thrombosis. It is a key regulatory molecule within the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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