Thermo Fisher Scientific PCNA Chimeric Recombinant Mouse Monoclonal Antibody (SAIC-27C-101)

Applications

Tested Dilution

Publications

Mass Spectrometry (MS)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, lambda

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SAIC-27C-101

Immunogen

Peptide LVQGSILK derived from PCNA1 (Proliferating cellular nuclear antigen 1) conjugated to KLH. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PCNA,uniProtId:P12004-1,ncbiNodeId:9606,antigenRange:6-13,antigenLength:261,antigenImageFileName:MA5-47907_PCNA_P12004-1_House_mouse.svg,antigenImageFileNamePDP:MA5-47907_PCNA_P12004-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074351

Product Specific Information

Specificity: Recognises human PCNA.

Preparation: Peptide LVQGSILK derived from PCNA1 (Proliferating cellular nuclear antigen 1) conjugated to KLH. PCNA ins an auxiliary and regulatory protein of DNA polymerase delta involved in control of eukaryotic DNA replication.

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.