Thermo Fisher Scientific ERK1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human ERK1 (MAPK3) for WB, IHC, IP applications. Recognizes residues 329–379 of ERK1. Supplied as unconjugated liquid at 0.2 mg/mL. Ideal for studying MAPK signaling and cell proliferation pathways.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 329 and 379 of human extracellular signal-related kinase 1 (MAPK3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that activates p90, RSK, MSK, ELK1, and Stat3, playing a key role in the MAPK signaling pathway. ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells.
It is activated by various stimuli including growth factors, cytokines, viral infection, and GPCR ligands, leading to nuclear translocation and phosphorylation of nuclear targets.
ERK1 participates in cellular processes such as proliferation, differentiation, and cell cycle progression. Alternative splicing results in different isoforms of ERK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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