Thermo Fisher Scientific ERK2 Polyclonal Antibody
ERK2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합합니다. 인간 ERK2의 310-360 잔기 영역을 면역원으로 제작되었으며, Rat과 Bovine에서도 반응이 예측됩니다. 4°C에서 보관하며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 310 and 360 of human extracellular signal-regulated kinase-2 (MAPK1). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway.
MAP kinases (ERKs) serve as integration points for multiple biochemical signals and are involved in cellular processes such as proliferation, differentiation, transcription regulation, and development.
Activation of this kinase requires phosphorylation by upstream kinases. Upon activation, ERK2 translocates to the nucleus, where it phosphorylates nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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