Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Super Bright 436, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Super Bright 436, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 4 publications

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV/Violet/Yellow series
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine series
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 600, 645, 702, 780
• NovaFluor Blue/Red/Yellow variants
• Request custom conjugation

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Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 forms a complex with CD132 (common gamma chain) to create the IL-7 receptor (IL-7R), a key molecule in lymphocyte development and homeostatic proliferation of T cells.

• CD127 is expressed by immature B cells, thymocytes, and most mature T cells.
• Binding of IL-7 triggers signal transduction via the Jak/STAT pathway.
• CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (Tregs), which can be used as a marker for suppressive Treg identification.
• Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (Product #14-1079-80).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells
Use at 5 µL (0.25 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)

Fluorochrome Information:
Super Bright 436 is excited with the violet laser (405 nm) and emits at 436 nm.
Recommended filter: 450/50 bandpass or equivalent.
Ensure instrument compatibility before use.

When using multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

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Target Information

CD127 (Interleukin-7 Receptor alpha, IL7R)

• Glycoprotein regulating lymphopoiesis
• Forms alpha/gamma heterodimer for active IL7R signaling
• Promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+/CD8+ T cells
• Associated diseases: Severe combined immunodeficiency (SCID), T cell negative/B cell negative/NK positive SCID

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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