Thermo Fisher Scientific TNF beta Polyclonal Antibody, PeproTech
TNF-beta에 특이적인 Rabbit Polyclonal Antibody로 인간 TNF-beta 검출용. Western blot, ELISA, Neutralization 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체. 연구용으로만 사용.
- 카탈로그번호
- 500-P31B-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TNF beta Polyclonal Antibody, PeproTech
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | - |
| Neutralization (Neu) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human TNF-beta |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930383 |
Product Specific Information
AA Sequence of recombinant protein:
MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TNF-beta. Anti-Human TNF-beta-specific antibody was purified by affinity chromatography employing an immobilized Human TNF-beta matrix.
Sandwich ELISA:
To detect Human TNF-beta by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TNF-beta (500-P31BBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human TNF-beta.
Western Blot:
To detect Human TNF-beta by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human TNF-beta is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. It is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta anchoring lymphotoxin-alpha to the cell surface. TNF beta mediates various inflammatory, immunostimulatory, and antiviral responses, contributes to secondary lymphoid organ formation during development, and plays a role in apoptosis. Genetic variations are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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