Thermo Fisher Scientific TNF beta Polyclonal Antibody, PeproTech

Applications

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TNF-beta. Anti-Human TNF-beta-specific antibody was purified by affinity chromatography employing an immobilized Human TNF-beta matrix.

Sandwich ELISA:
To detect Human TNF-beta by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TNF-beta (500-P31BBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human TNF-beta.

Western Blot:
To detect Human TNF-beta by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human TNF-beta is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. It is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta anchoring lymphotoxin-alpha to the cell surface. TNF beta mediates various inflammatory, immunostimulatory, and antiviral responses, contributes to secondary lymphoid organ formation during development, and plays a role in apoptosis. Genetic variations are associated with susceptibility to leprosy type 4 and psoriatic arthritis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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