Thermo Fisher Scientific CD40 Monoclonal Antibody (OTI7H1)

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD40 is a single-chain glycoprotein belonging to the tumor necrosis factor/nerve growth factor superfamily, showing homology to the Hodgkin’s disease-associated antigen CD30.
It is expressed on all B cells except plasma cells, as well as on some epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 plays a key role in immune and inflammatory responses, including:

• T cell-dependent immunoglobulin class switching
• Memory B cell development
• Germinal center formation

AKNA transcription factor regulates CD40 expression, influencing cell interactions.
Adaptor protein TNFR2 interacts with CD40, mediating signal transduction.
The CD40-CD40L interaction is implicated in amyloid-beta-induced microglial activation, an early event in Alzheimer’s disease pathogenesis.
Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
CD40 dysfunction is associated with Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.