
Thermo Fisher Scientific MIP-3 alpha Polyclonal Antibody
Human MIP-3 alpha(CCL20)에 특이적인 Goat Polyclonal Antibody로, WB, IHC, ICC, Neutralization에 적합. 항원 친화 크로마토그래피로 정제되었으며, Lyophilized 형태로 제공. PBS 및 trehalose buffer에 용해 가능하며, -20°C에서 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | - |
| Neutralization (Neu) | 0.2–1.0 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human CCL20/MIP-3 alpha (Ala27–Met96, Asn95Asp) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608263 |
Product Specific Information
- In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse Teck is observed, and less than 1% cross-reactivity with recombinant human MIP-3 beta is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of antibody (LAL method).
Target Information
Macrophage Inflammatory Protein-3 alpha (MIP-3α), also known as CCL20, is expressed in the liver, lungs, lymph nodes, and peripheral blood lymphocytes. MIP-3α is upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10. It signals through the G protein-coupled receptor CCR6 and acts as a chemoattractant for lymphocytes and dendritic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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