Thermo Fisher Scientific RGMB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RGM-B Gly49-Ser414 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RGMB,uniProtId:Q7TQ33-1,ncbiNodeId:10090,antigenRange:49-414,antigenLength:436,antigenImageFileName:PA5-47516_RGMB_Q7TQ33-1_Sheep.svg,antigenImageFileNamePDP:PA5-47516_RGMB_Q7TQ33-1_Sheep_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607083

Product Specific Information

In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) RGM-A and rmRGM-C is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Repulsive Guidance Molecule B (RGMB) is primarily expressed in the central nervous system. RGM A and B both appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGMA appears in the neuroepithelium, and only RGMB in the peripheral nervous system. During development, both RGMA and B appear also in lung and in limb cartilage, while RGMB has additional expression domains in pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.