Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI3F3), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and participates in DNA excision and mismatch repair pathways. It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA is a marker of proliferative potential and is used to assess tumor proliferation status. It localizes to the nucleus, acts as a cofactor of DNA polymerase delta, and forms a homotrimer that enhances DNA synthesis processivity. Upon DNA damage, PCNA is ubiquitinated and functions in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.