Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), APC-eFluor 780, eBioscience
마우스 CD69 단백질을 인식하는 H1.2F3 단클론 항체로, APC-eFluor 780 형광체가 결합된 제품입니다. 유세포 분석용으로 최적화되었으며, 0.5 µg/test 농도로 사용 가능합니다. T 및 B 세포 활성화 연구에 적합하며, 4°C 암조건에서 보관합니다.
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- 47-0691-8x (2개 옵션)
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Applications
- Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- View 56 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H1.2F3 |
| Conjugate | APC-eFluor™ 780 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573965 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 496
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 786
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer on lymphocytes. Activation of T and B cells rapidly upregulates CD69 expression, indicating its role in lymphocyte development and activation.
Applications Reported:
- Flow cytometric analysis
Applications Tested:
- Tested on stimulated mouse splenocytes
- Recommended ≤ 0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
- Optimize antibody titration for best performance
Fluorophore Information:
- APC-eFluor 780 emits at 780 nm, excited by Red laser (633 nm)
- Ensure instrument compatibility
- Light sensitive: protect from photo-oxidation
Fixation:
Samples may be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product #00-8222)
- 1-step Fix/Lyse Solution (Product #00-5333)
Excitation/Emission: 633–647 nm / 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD69 (AIM, Active Inducer Molecule):
CD69 is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains N-linked oligosaccharides and is expressed on activated platelets. In peripheral blood, CD69 expression varies and is involved in lymphocyte signal transduction. Expression is induced upon T cell activation and may play a role in proliferation and signal transmission in NK cells and platelets. Alternative splicing yields multiple transcript variants.
Associated Diseases:
- Coccidiodomycosis
- Asthma
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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