Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), APC-eFluor 780, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 0.5 µg/test
  • View 56 publications

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Product Specifications

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Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

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Product Specific Information

Description:
The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer on lymphocytes. Activation of T and B cells rapidly upregulates CD69 expression, indicating its role in lymphocyte development and activation.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested on stimulated mouse splenocytes
• Recommended ≤ 0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimize antibody titration for best performance

Fluorophore Information:

• APC-eFluor 780 emits at 780 nm, excited by Red laser (633 nm)
• Ensure instrument compatibility
• Light sensitive: protect from photo-oxidation

Fixation:
Samples may be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)

Excitation/Emission: 633–647 nm / 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

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Target Information

CD69 (AIM, Active Inducer Molecule):
CD69 is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains N-linked oligosaccharides and is expressed on activated platelets. In peripheral blood, CD69 expression varies and is involved in lymphocyte signal transduction. Expression is induced upon T cell activation and may play a role in proliferation and signal transmission in NK cells and platelets. Alternative splicing yields multiple transcript variants.

Associated Diseases:

• Coccidiodomycosis
• Asthma

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For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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