Thermo Fisher Scientific EAAT1 (GLAST) (extracellular) Polyclonal Antibody
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EAAT1(GLAST) 단백질의 세포외 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC(F), ICC/IF, Flow Cytometry에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 동결건조 형태. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)KQFKTSYEKRSFK, corresponding to amino acids 188–200 of rat EAAT1, 2nd extracellular loop |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: Add 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
- The antibody ships as a lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For long-term storage, aliquot and keep at -20°C.
- Avoid multiple freeze/thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
This gene encodes a member of the high-affinity glutamate transporter family. Mutations in this gene are associated with episodic ataxia type 6. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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