Thermo Fisher Scientific Alkaline Phosphatase Polyclonal Antibody
Thermo Fisher Scientific의 Alkaline Phosphatase Polyclonal Antibody는 소(칼프) 장 알칼리성 포스파타아제에 특이적으로 반응하는 토끼 IgG 항체입니다. WB, IHC, ELISA, IP 등 다양한 응용에 적합하며, 고순도 이온 교환 크로마토그래피로 정제되었습니다. 연구용 전용 제품입니다.
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Thermo Fisher Scientific Alkaline Phosphatase Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Alkaline Phosphatase (Calf Intestine) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase (Calf Intestine).
Cross-reactivity against Alkaline Phosphatase from other tissues and species may occur but has not been specifically determined.
Target Information
Alkaline phosphatase is a homodimeric enzyme (~86 kDa) containing two zinc atoms per monomer, essential for catalytic function, and is optimally active at alkaline pH.
It is found in both prokaryotes and eukaryotes, performing similar functions with structural adaptations to each organism.
ALP plays a role in liver metabolism and skeletal development, acts as a source of inorganic phosphate in bacteria, and serves as a useful protein for studying membrane protein sorting in yeast.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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